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Ethylene signaling induces gelatinous layers with typical features of tension wood in hybrid aspen 2018 (Engelska)Ingår i: New Phytologis, Vol. 218, nr 3, s. 999-1014Artikel i tidskrift (Refereegranskat) Published

Abstract [en]

• The phytohormone ethylene impacts secondary stem growth in plants by stimulating cambial activity, xylem development and fiber over vessel formation.
• We report the effect of ethylene on secondary cell wall formation and the molecular connection between ethylene signaling and wood formation. We applied exogenous ethylene or its precursor 1-aminocyclopropane-1-carboxylic acid (ACC) to wild-type and ethylene-insensitive hybrid aspen trees (Populus tremulaxtremuloides) and studied secondary cell wall anatomy, chemistry and ultrastructure. We furthermore analyzed the transcriptome (RNA Seq) after ACC application to wild-type and ethylene-insensitive trees.
• We demonstrate that ACC and ethylene induce gelatinous layers (G-layers) and alter the fiber cell wall cellulose microfibril angle. G-layers are tertiary wall layers rich in cellulose, typically found in tension wood of aspen trees. A vast majority of transcripts affected by ACC are downstream of ethylene perception and include a large number of transcription factors (TFs). Motif-analyses reveal potential connections between ethylene TFs (Ethylene Response Factors (ERFs), ETHYLENE INSENSITIVE 3/ETHYLENE INSENSITIVE3-LIKE1 (EIN3/EIL1)) and wood formation.
• G-layer formation upon ethylene application suggests that the increase in ethylene biosynthesis observed during tension wood formation is important for its formation. Ethylene-regulated TFs of the ERF and EIN3/EIL1 type could transmit the ethylene signal.

Ort, förlag, år, upplaga, sidor

Wiley-Blackwell, 2018. Vol. 218, nr 3, s. 999-1014

Nyckelord [en]

cell wall, ethylene signaling, gelatinous layer (G-layer), hybrid aspen, tension wood, transcriptome

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Identifikatorer

URN: urn:nbn:se:umu:diva-138586DOI: 10.1111/nph.15078ISI: 000430127000016PubMedID: 29528503OAI: oai:DiVA.org:umu-138586DiVA, id: diva2:2905

Forskningsfinansiär

Forskningsrådet Formas, 213-2011-1148Forskningsrådet Formas, 239-2011-1915Tillgänglig från: 2018-06-26 Skapad: 2018-06-26 Senast uppdaterad: 2018-06-26Bibliografiskt granskad

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Av författaren/redaktören

Delhomme, NicolasHvidsten, Torgeir R.

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Umeå Plant Science Centre (UPSC)Kemiska institutionen

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